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J. Biol. Chem. 272, 23489-23497. Transcriptional regulation of the mouse presenilin-1 gene. 1997

Mitsuda, N., Roses, A.D. and Vitek, M.P.

Notes: The Dual-Luciferase™ Reporter System was used to quantitate the presenilin promoter activity in Neuro2a neuroblastoma cells, mouse P19 embryonal carcinoma cells and NIH 3T3 cells. Studies were also performed in P19 cells treated with retinoic acid to acquire a neuron-like phenotype and P19 cells treated with dimethyl sulfoxide to acquire a muscle-like phenotype. The presenilin promoter functioned best in the Neuro2a and neuron-like P19 cells. 5´-RACE products from mouse brain RNA were purified with the Wizard^® PCR Preps System and cloned into the pGEM-T Vector . The cloned amplimers were sequenced and used as a template for amplification to produce truncation mutants to assess promoter activity. (1590)

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Dual-Luciferase® Reporter Assay System

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J. Biol. Chem. 272, 13019-13025. TrkB variants with deletions in the leucine-rich motifs of the extracellular domain. 1997

Ninkina, N. , Grashchuck, M. , Buchman, V. L. , Davies, A. M.

Notes: The PolyATtract^® System 1000 was used to isolate polyA+ RNA directly from newborn mouse brains. The RNA was used in RT-PCR for detection of specific TrkB variants. Taq DNA Polymerase and the pGEM^®-T Vector System were used to produce and subclone novel TrkB variants that differ in the extracellular domain. The CellTiter 96^® Non-Radioactive Cell Proliferation Assay was performed on NIH 3T3 cells transfected with the TrkB receptors and challenged with BDNF, NT3 and NT4/5. (0615)

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CellTiter 96® Non-Radioactive Cell Proliferation Assay

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Proc. Natl. Acad. Sci. USA 93, 13020-13023. Pinus banksiana has at least seven expressed alcohol dehydrogenase genes in two linked groups. 1996

Perry, D. and Furnier, G.

Notes: The pGEM^®-T Vector System was used in this study to clone amplified cDNAs. (1973)

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pGEM®-T Vector System I

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J. Clin. Invest. 98(1), 43-49. Acute regulation by insulin of phosphatidylinositol-3-kinase, Rad, Glut 4, and lipoprotein lipase mRNA levels in human muscle. 1996

Laville, M., Auboeuf, D., Khalfallah, Y., Vega, N., Riou, J.P. and Vidal H.

Notes: Promega's Tth DNA Polymerase, pGEM^®-T Vector System and Riboprobe^® System were used in this study. (2002)

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pGEM®-T Vector System I

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Proc. Natl. Acad. Sci. USA 93, 14548-14553. Characterization of serum amyloid A protein mRNA expression and secondary amyloidosis in the domestic duck. 1996

Guo, J., Aldrich, C., Mason, W. and Fox, J.

Notes: The pGEM^®-T Vector System was used in this study. (2194)

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pGEM®-T Vector System I

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Proc. Natl. Acad. Sci. USA 93, 13036-13041. Evolutionary analyses of hedgehog and Hoxd-10 genes in fish species closely related to the zebrafish. 1996

Zardoya, R. , Abouheif, E. , Meyer, A.

Notes: PCR products were cloned into the pGEM^®-T Vector System. (0082)

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pGEM®-T Vector System I

pGEM®-T Vector System II

J. Mol. Neurosci. 7, 193-201. Four repeat high-mol-wt MAP2 forms in rat dorsal root ganglia. 1996

Forleo, P., Couchie, D., Chabas, S. and Nunez, J.

Notes: The system was used to clone the amplimers generated by RT-PCR. Sequencing of the clones was performed in the vector. (1569)

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pGEM®-T Vector System I

pGEM®-T Vector System II

Proc. Natl. Acad. Sci. USA 93, 15244-15248. Independent evolution of the prochlorophyte and green plant chlorophyll a/b light-harvesting proteins. 1996

Roche, J. van der Staay, G., Partensky, F., Ducret, A., Aebersold, R., Li, R., Golden, S., Hiller, R., Wrench, R., Larkum, A. and Green, B.

Notes: The pGEM^®-T Vector System was used in this study. (2005)

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pGEM®-T Vector System I

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Proc. Natl. Acad. Sci. USA 93, 13014-13019. Inhibition of granulocytic differentiation by mNotch1. 1996

Milner, L., Bigas, A., Kopan, R., Brashem-Stein, C., Bernstein, I. and Martin, D.

Notes: The pGEM^®-T Vector System was used in this study. (1970)

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pGEM®-T Vector System I

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Proc. Natl. Acad. Sci. USA 93, 14972-14977. Isolation and characterization of a tobacco mosaic virus-inducible myb oncogene homolog from tobacco. 1996

Yang, Y. and Klessig, D.F.

Notes: The pGEM^®-T Vector System was used to clone PCR products amplified from a tobacco cDNA library. (1968)

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pGEM®-T Vector System I

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Proc. Natl. Acad. Sci. USA 93, 15394-15399. Prevention of hepatitis C virus infection in chimpanzees by hyperimmune serum against the hypervariable region 1 of the envelope 2 protein. 1996

Farci, P., Shimoda, A., Wong, D., Cabezon, T., DeGioannis, D., Strazzera, A., Shimizu, Y., Shapiro, M., Alter, H., Purcell, R.

Notes: The pGEM^®-T Vector System was used in this study. (2193)

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pGEM®-T Vector System I

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Proc. Natl. Acad. Sci. USA 93, 12885-12889. RGS-r, a retinal specific RGS protein, binds an intermediate conformation of transducin and enhances recycling. 1996

Chen, C., Wieland, T., Simon, M.

Notes: The pGEM^®-T Vector System was used in this study. (1967)

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pGEM®-T Vector System I

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Cell 87, 1181-1190. Vascular dysmorphogenesis caused by an activating mutation in the receptor tyrosine kinase TIE2 1996

Vikkula, M. , Boon, L. M. , Carraway, K. L. , Calvert, J. T. , Diamonti, A. J. , Goumnerov, B. , Pasyk, K. A. , Marchuk, D. A. , Warman, M. L. , Cantley, L. C. , Mulliken, J. B. , Olsen, B. R.

Notes: A 3,375 bp fragment was cloned in the pGEM^®-T Vector System. (0223)

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pGEM®-T Vector System II

pGEM®-T Vector System I

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