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Cryobiology 31(5), 468-477. Effects of cryopreservation on immune response: VII. Freezing induced enhancement of IL-6 production in human peripheral blood mononuclear cells. 1994

Venkataraman, M.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS) was used to perform IL-6 bioassays using the B9 cell line. (1715)

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Methods in Neuroscience 22(Chap. 31), 510-522. Steroids and central regulation of immune response. 1994

Shöbitz, B.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS) was used to do IL-6 bioassays in this chapter. The author references Promega technical bulletin number 169 and shows a figure comparing MTS to tritiated thymidine incorporation. (2234)

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Parasitol. Res. 80, 235-239. The use of a water-soluble formazan complex to quantitate the cell number and mitochondrial function of Leishmania major promastigotes. 1994

Berg, K., Zhai, L., Chen, M., Kharazmi, A. and Owen, T.C.

Notes: The authors demonstrate that MTS measurements can be used to determine number of parasites. Results using the MTS method were confirmed by radioactive thymidine uptake and cell counting. They concluded the MTS method (CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay) was simple, fast, highly reproducible, and suitable for drug screening, identification of drug resistant isolates and growth kinetics studies. (1494)

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Kurume Med. J. 41(4), 165-169. Transformation assays of the cell mitotic and proliferation activities of purified oncogene products. 1994

Yuge, K., Chinami, M., and Shingu, M.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay and 3H-thymidine incorporation were used to measure the effect of introducing three E7 proteins by osmotic shock on the metabolic activities of C127 mouse fibroblast cells. The degrees of change among the three E7 proteins were similar confirming that the CellTiter 96® AQueous Assay could be used to replace 3H-thymidine incorporation in this system. (1729)

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Proc. Natl. Acad. Sci. USA 90, 572-576. Ciliary neurotrophic factor coordinately activates transcription of neuropeptide genes in a neuroblastoma cell line. 1993

Symes, A.J., Rao, M.S., Lewis, S.E., Landis, S.C., Hyman, S.E. and Fink, J.S.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used to test the effects of CNTF on NBFL human neuroblastoma cells. The study reports that while gene expression is altered, no alteration in cell number or morphology were detectable. (1531)

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Nat. Toxins. 1(5), 303-307. Rapid colorimetric bioassay for screening of Fusarium mycotoxins. 1993

Rotter, B.A., Thompson, B.K., Clarkin, S., and Owen, T.C.

Notes: MTT and MTS/PMS assays were used to measure cytotoxicity of Fusarium toxins on BHK-21, baby hamster kidney cells. The inherent disadvantage of the MTT assay (formation of insoluble formazan) was overcome by using MTS and measuring the water soluble formazan directly in the culture media. The replacement of MTT by MTS substantially reduced the number of sample processing steps and the length of time required to complete the cytotoxicity assay. (1701)

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Mol. Biol. Cell 3(Suppl.), 184a. Comparison of MTT, XTT, and a novel tetrazolium compound MTS for in vitro proliferation and chemosensitivity assays. 1992

Riss, T.L. and Moravec. R.A.

Notes: This abstract describes the characterization of the MTS proliferation assay and makes a comparison to MTT and XTT tetrazolium compounds. This is the reference to a poster Promega presented at the American Society for Cell Biology and is available as Promega literature part number RP006. (2086)

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