Notes: The 32D cell line was transfected with the colony stimulated factor-1 receptor. The cells were cultured with either IL-3 or CSF-1 with various amounts of dibutryl-cAMP or forskolin. The CSF-1 treated cells proliferated more closely to untreated cells than the IL-3-treated cells. Proliferation was measured with the CellTiter 96^® AQ_ueous Assay (MTS/PMS). (0807)

Notes: MDCK and HEK 293 cells were transfected with DNA encoding the beta subunit of the Drosophila homolog of the insulin receptor or with DNA encoding the subunit with the C-terminal extension deleted. The authors used the CellTiter^® 96 AQ_ueous Non-Radioactive Cell Proliferation Assay to assess the effect of expression of these two constructs on cell growth. (2501)

Notes: The CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to determine the amount of Jurkat cells that had migrated through a chemotaxis chamber. (0357)

Notes: The presence of type II TGFβ receptor mutations in 12 colorectal cancer cell lines (LS174T, HCA7, LOVO, SW48, DLD1, HCT116, COLO201, COLO205, COLO320, HCA46, HT29, and CACO2) was examined. Six cell lines were found to have homozygous mutations. Growth inhibition upon TGFβ1 stimulation and changes in adhesion molecule expression of these cell lines was also studied. Cells were stimulated with Promega's human TGFβ1 at a concentration of 2.5 ng/ml and cell proliferation was measured using the CellTiter 96^® AQ_ueous Nonradioactive Cell Proliferation Assay. Two cell lines were shown to be inhibited by TGFβ1 despite homozygous mutations in the type II TGFβ receptor gene. As a control, TGFβ1 was bioneutralized with the Anti-TGFβ1 pAb at a 2:1 molar ratio of antibody to TGFβ1. (2457)

Notes: Contribution of the EWS/ATF1 chimeric protein to tumor phenotype was investigated by expressing an inhibitory anti-AFT1 single chain antibody in cells derived from clear cell sarcoma. Cell viability was monitored using the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay. (2543)

Notes: Various lipid formulations were used to transfect NIH 3T3 cells with the pGL3 Control Vector to monitor the transfection. The cytotoxic effect of these lipid combinations were measured with the CellTiter 96^® AQ_ueous Proliferation Assay (MTS/PMS). (2054)

Notes: The CellTiter^® 96 AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to monitor the viability of human umbilical vein epithelial cells and bovine aortic endothelial cells following treatment with various pharmacological reagents. (0163)

Notes: The authors investigated the effect of the glucocorticoid, dexamethasone, on the proliferation of primary cultures of rat mammary fibroblasts (RMF) using the  CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay. (2488)

Notes: The authors used CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) with human and mouse glioma cells (0987)

Notes: HUVECs were stimulated with either recombinant angiopoetin-1 or -2 or VEGF, and cell proliferation was assayed using the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay. (2511)

Notes: The CellTiter 96^® AQ_ueous Cell Proliferation Assay was used to determine the viability of Jurkat, BJAB, HT-29, A20 and WEHI164  cells in response to various soluble factors like soluble Fas Ligand and soluble TNF-related apoptosis-inducing ligand. Antibody-induced aggregation of the ligand increased the cytotoxicity. Good detail is provided for the assay. (0413)

Notes: The CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to demonstrate that the cyclosporin treatment had no cytotoxic effect on the Cryptosporidium parvum host cell, Caco-2 cells. (0560)

Notes: WT19 (myeloblast-like mouse cells) expressing the α-subunit along with wildtype or mutant β-subunit of the GM-CSF receptor were incubated with various concentrations of human GM-CSF and cell proliferation was assayed using the CellTiter 96^® AQ_ueous Cell Proliferation Assay. (2554)

Notes: The authors investigated the mechanism through which the extracellular matrix proteoglycan, decorin, inhibits cell cycle progression. The CellTiter 96® AQ_ueous Non-Radioactive Cell Proliferation Assay was used to determine growth curves for A431 squamous carcinoma cells transfected with a variety of decorin clones. The assay was also used to determine the effects of exogenous human recombinant decorin, decorin protein or biglycan on wildtype A431 cells. The authors also measured the ability of tyrphostin AG1468 to prevent the growth-inhibitory effects of decorin in A431 using the same assay. (2475)

Notes: The MTS-based CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay was used to follow the survival of HL-60/ADR cells to the cytotoxic agent, vincristine. Various compounds were tested to inhibit the multidrug resistance protein-mediated survival of the cells. (1501)

Notes: The CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to measure the proliferation of Neuro2a cells in response to various peptides. The cells were plated at 8000 cell/well 24hr prior to addition of the peptides. Forty-eight hours later, the media was removed new media added and the proliferation assay performed. (0826)

Notes: The cytotoxicities of nitaoxanide and paromomycin to MDBK cells were assessed using the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay. (2529)

Notes: To assay for activation-induced cell death, Jurkat cells were plated in wells coated with anti-human CD3 antibodies. Cell viability was assessed using the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay. (2512)

Notes: L6.G8 cells were seeded in 96-well plates and grown to confluence and then incubated with hemin or sodium nitroprusside for 6 hours. Cell viability was assessed using the CellTiter 96® AQ_ueous Non-Radioactive Cell Proliferation Assay. (2520)

Notes: The CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay was used to assess changes in viable cell mass of SNU-C1, SNU-C4 and SNU-C5 colon cancer cell lines following treatment with EGFR tyrosine kinase inhibitors. (0961)

Notes: Four adult T-cell leukemia cell lines were cultured with rIL-2, and cell proliferation was assessed using the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay. (2519)

Notes: Cell growth of human hepatoma cell lines, HCC-M and HCC-T was measured using the CellTiter 96^® AQ_ueous Non-Radioactive Cell Proliferation Assay. (2516)

Notes: The CellTiter^® 96 AQ_ueous Cell Proliferation Assay (MTS/PMS) was used to assess the proliferative activity of Pleiotrophin and Midkine on NIH3T3, normal rat kidney cells, and the G401 kidney rhabdoid tumor cells. The assay was also used to assay the mitogenic activity of Pleiotrophin and midkine on G401 cells from 4-120 hours. (0501)

Notes: Thrombopoietin (TPO)  is a cytokine that regulates megakaryocytopoiesis and thrombopoiesis. These authors raised antibodies to peptides derived from TPO and rhTPO in order to characterize immunologically distinct regions of TPO. They assessed the ability of the antibodies to interfere with TPO-stimulated cell proliferation of FDCP-2 cells that constitutively express human Mpl, the receptor for TPO, using the CellTiter 96® AQ_ueous Non-Radioactive Cell Proliferation Assay. They show dose-dependent inhibition of rhTPO-induced growth of these cells by specific antibodies. (2486)

Notes: The CellTiter 96® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to monitor the viability of various breast cancer cell line following treatment with taxol. (0119)