Notes: This paper discusses the inhibition of PCR by reverse transcriptases. The inhibitory effect was overcome by increasing template concentration to more than 10^5 to 10^6 copies or including T4 gene 32 protein during the reverse transcription phase of the reaction. The poly(A)+ RNA used in the study was isolated using the PolyATtract® mRNA Isolation System. (1357)

Notes: The authors used the PolyATtract® mRNA Isolation System to isolate mRNA from total RNA. They used this mRNA-rich fraction for primer extension analysis using AMV Reverse Transcriptase. The Wizard® Plus Midipreps DNA Purification System was used for various plasmid isolations. The Dual-Luciferase® Reporter Assay System was used to study promoters cloned into the pGL3-Basic Vector. The pRL-SV40 was used as a transfection control plasmid. (0095)

Notes: Poly A+ RNA was isolated from Drosophila head total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for the production of subtraction cDNA libraries. (1685)

Notes: PolyA+ RNA was isolated from I. balsamina seed total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for cDNA synthesis. (1689)

Notes: PolyA+ RNA was isolated from Drosophila yakuba embryo total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for cDNA library construction. (0410)

Notes: PolyA+ RNA was isolated from Drosophila yakuba embryo total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for cDNA library construction. (1687)

Notes: Poly A+ RNA was isolated from D. salina total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for cDNA library construction. (1722)

Notes: PolyA+ RNA was isolated from secondary normal human kidney cell total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for Northern analysis. (1724)

Notes: In this study, total RNA was isolated from frozen tumor specimens and cell lines using the RNAgents^® Total RNA Isolation System. The PolyATtract^® mRNA Isolation System was used to isolate Poly(A)+ RNA from B cell lymphocytic leukemia cell total RNA. (2027)

Notes: Poly A+ RNA was isolated from the total RNA of nine different human cell lines using the PolyATtract^® mRNA Isolation System. The RNA was examined by Northern analysis. (1696)

Notes: The PolyATtract^® mRNA Isolation System IV was used to isolate poly(A)+ RNA from Anopheles gambiae (mosquito) total RNA. The isolated RNA was used for cDNA synthesis using Promega's Universal Riboclone^® cDNA Synthesis System. (0498)

Notes: The PolyATtract^® mRNA Isolation System was used to isolate poly A+ RNA from Anopheles gambiae (mosquito) total RNA. The isolated RNA was used for cDNA synthesis with the Universal Riboclone System. (1683)

Notes: Poly A+ RNA was isolated from ES and 4-3+ cells. Unclear whether starting material was cells or total RNA. The isolated polyA+ RNA was used for Northern analysis. (1684)

Notes: The PolyATtract^® mRNA Isolation System was used to isolate poly(A)⁺ RNA from total adult Drosophila RNA. The isolated RNA was used for Northern analysis. (1111)

Notes: PCR products were directly sequenced with the fmol^® DNA Cycle Sequencing System using the end-labeled primer protocol. (0739)

Notes: Poly(A)+ RNA was isolated from RIN rat insulinoma cell total RNA with PolyATtract® mRNA Isolation System III. (1264)

Notes: Poly(A+) RNA was isolated from RIN rat insulinoma cell total RNA and was used for Northern blots. The authors used Promega's PolyATtract^® mRNA Isolation System and AMV Reverse Transcriptase in this study. (2107)

Notes: The PolyATtract^® mRNA Isolation System was used to isolate polyA+ RNA from total human liver RNA. The isolated RNA was used for primer extension analysis. (1697)

Notes: The PolyATtract^® mRNA Isolation System II was used to isolate polyA+ RNA from total human liver RNA. The isolated RNA was used for primer extension analysis. (0159)

Notes: The PolyATtract^® mRNA Isolation System was used to isolate poly(A)+ RNA from barley total RNA. The isolated RNA was used for Northern analysis. (1299)

Notes: In this study, Poly(A)+ RNA was isolated from B16 melanoma cell total RNA using the PolyATtract^® mRNA Isolation System. The isolated RNA was used for cDNA library construction. The RNAgents^® Total RNA Isolation System was used to isolate total RNA from HEL and K562 human erythroleukemia cells. The isolated RNA was used for RT-PCR. RNasin^® Ribonuclease Inhibitor was used in a very innovative fashion. Peripheral mononuclear cells isolated from blood were directly lysed in a hypotonic buffer containing the inhibitor. The lysates from these cells were used directly for RT-PCR. (2026)

Notes: Poly(A) RNA was isolated using the PolyATtract^® mRNA Isolation System and used to create a library in a mammalian expression vector. Pools of 25 colonies of E.coli clones were used to transfect 293 cells in a limited dilution screen. (2044)

Notes: Poly A+ RNA was isolated from the protozoan Leishmania mexicanna amazonesis total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM-T Vector. (1678)

Notes: PolyATtract^® mRNA Isolation System was used to isolate Poly A+ RNA from the protozoan Leishmania mexicanna amazonesis total RNA. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM^®-T Vector. (0748)

Notes: The PolyATtract^® mRNA Isolation System was used to isolate polyA+ RNA from mouse spleen total RNA. The isolated RNA was used for cDNA synthesis. (1725)