J. Biol. Chem.
Caspase cleavage of gene products associated with triplet expansion disorders generates truncated fragments containing the polyglutamine tract.
Wellington, C.L., Ellerby, L.M., Hackam, A.S., Margolis, R.L., Trifiro, M.A., Singaraja, R., McCutcheon, K., Salvesen, G.S., Propp, S.S., Bromm, M., Rowland, K.J., Zhang, T., Rasper, D., Roy, S., Thornberry, N., Pinsky, L., Kakizuka, A., Ross, C.A., Nicholson, D.W., Bredesen, D.E., and Hayden, M.R.
Notes: The TNT® Coupled Wheat Germ Extract and the TNT® Coupled Rabbit Reticulocyte Lysate Systems were used to in vitro express and 35S-methionine label various Huntington protein mutants and polyglutamine-containing proteins. These proteins included a full-length 210KDa Huntington, a 210KDa Atrophin-1, and a 140KDa Androgen Receptor protein. The expressed proteins were incubated with apoptotic extracts or purified caspases to detect cleavage by caspases. Mutated Huntington constructs were cloned into the pCI-neo Vector. Data from autoradiographs were quantitated by laser band densitometry. (3019)
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