HaloTag® technology is a powerful tool for functional protein analysis based on the formation of a covalent bond between the HaloTag® protein fusion tag and synthetic chemical ligands. By interchanging ligands, you can control the function and properties of the HaloTag® fusion protein, allowing use of the same construct for protein purification, protein interaction analysis, and cell-based assays for protein translocation, receptor internalization, and protein stability studies.
Available HaloTag® ligands include magnetic and non-magnetic beads, glass slides, cell-permeable and impermeable fluorescent ligands, biotin, and reactive ligands that allow you to build your own custom HaloTag® ligands and surfaces.
In addition, HaloTag® technology allows researchers to image live cells, perform multicolor imaging experiments and immunocytochemistry, or potentially immunohistochemistry. See the Cell Imaging Protocols & Applications Guide chapter for details.