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New High-Throughput Technology for Screening Transcriptional Regulatory Elements and Gene Networks

Nathan D. Trinklein and Shelley Force Aldred
SwitchGear Genomics, Menlo Park, CA
Publication Date: 2007

Abstract

Here we describe the development of a new set of tools that enable detailed analysis of regulatory pathways in living cells. SwitchGear Genomics has produced a library of thousands of human promoters, UTRs and other regulatory elements contained within a state-of-the-art luciferase reporter vector, the pGL4.11[luc2P] Vector. This library combines the ability to gather highly reproducible data over a broad dynamic range with the ability to scale to hundreds or thousands of promoters in a single experiment.

Promega Notes 96, 8–10.