HaloTagâ„¢ Interchangeable Labeling Technology for Cell Imaging Protein Capture and Immobilization
1Promega Corporation, 2Promega Biosciences, Incorporated
Publication Date: 2005
Abstract
The HaloTagâ„¢ Interchangeable Labeling Technology is designed to provide new options for rapid, site-specific labeling of proteins in living cells and in vitro. The technology is based on the efficient formation of a covalent bond between the HaloTagâ„¢ Protein and synthetic ligands. The covalent bond forms rapidly under physiological conditions, is highly specific and essentially irreversible, yielding a stable complex even under denaturing conditions. The HaloTagâ„¢ Ligand can carry a variety of functionalities, including fluorescent labels, affinity handles and attachments to a solid phase. The flexibility to create labeled HaloTagâ„¢ fusion proteins with a wide range of optical properties and functions will allow researchers to image and localize labeled HaloTagâ„¢ Protein fusions in live- or fixed-cell populations as well as isolate and analyze HaloTagâ„¢ Protein fusions and protein complexes.
Promega Notes 89, 2–6.
An introduction to HaloTag®--a versatile and powerful protein technology.
