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HaloTag™ Interchangeable Labeling Technology for Cell Imaging Protein Capture and Immobilization

Georgyi V. Los1, Al Darzins1, Chad Zimprich1, Natasha Karassina1, Randall Learish1, Mark G. McDougall2, Lance P. Encell1, Rachel Friedman-Ohana1, Monika Wood1, Gediminas Vidugiris1, Kris Zimmerman1, Paul Otto1, Dieter H. Klaubert2 and Keith Wood1
1Promega Corporation, 2Promega Biosciences, Incorporated
Publication Date: 2005

Abstract

The HaloTag™ Interchangeable Labeling Technology is designed to provide new options for rapid, site-specific labeling of proteins in living cells and in vitro. The technology is based on the efficient formation of a covalent bond between the HaloTag™ Protein and synthetic ligands. The covalent bond forms rapidly under physiological conditions, is highly specific and essentially irreversible, yielding a stable complex even under denaturing conditions. The HaloTag™ Ligand can carry a variety of functionalities, including fluorescent labels, affinity handles and attachments to a solid phase. The flexibility to create labeled HaloTag™ fusion proteins with a wide range of optical properties and functions will allow researchers to image and localize labeled HaloTag™ Protein fusions in live- or fixed-cell populations as well as isolate and analyze HaloTag™ Protein fusions and protein complexes.

Promega Notes 89, 2–6.

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