Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Achieve the Protein Expression Level You Need with the Mammalian HaloTag 7 Flexi Vectors

Michael Slater, Danette Hartzell, Jim Hartnett, Sarah Wheeler, Pete Stecha, and Natasha Karassina
Promega Corporation
Publication Date: 2008


A series of vectors containing deletions of human cytomegalovirus (CMV) immediate-early enhancer/promoter were created to provide different constitutive expression levels of HaloTag 7 fusion proteins, providing greater control over the level of protein expression. This allows expression of enough fusion protein to image or capture but not so much to disturb normal function of the protein partner fused to the HaloTag 7 protein.

Promega Notes 100, 16–18.