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Flexi® Vector Systems Technical Manual

Instructions for Use of Product(s)
C8640, C8820, C9320
Literature # TM254

The Flexi® Vector Systems use two rare-cutting restriction enzymes, SgfI and PmeI, in a simple, yet powerful, directional cloning method for protein-coding sequences. These systems provide a rapid, efficient and high-fidelity way to transfer these sequences between a variety of Flexi® Vectors. The Flexi® Vectors contain various expression or peptide tag options to enable expression of native or fusion proteins to study protein structure and function, as well as protein-protein interactions. All Flexi® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for successful ligation of the insert.

Summary of Changes
The following changes were made to the 5/17 revision of this document:
1. The web address for the Flexi® Vector Primer Design Tool was updated.
2. The document design was updated.

Printed in USA. Revised 5/17

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