Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Flexi® Vector Systems Technical Manual

Instructions for Use of Product(s)
C8640, C8820, C9320

Literature # TM254

The Flexi® Vector Systems use two rare-cutting restriction enzymes, SgfI and PmeI, in a simple, yet powerful, directional cloning method for protein-coding sequences. These systems provide a rapid, efficient and high-fidelity way to transfer these sequences between a variety of Flexi® Vectors. The Flexi® Vectors contain various expression or peptide tag options to enable expression of native or fusion proteins to study protein structure and function, as well as protein-protein interactions. All Flexi® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for successful ligation of the insert.

Summary of Changes
The following changes were made to the 10/21 revision of this document:
1. Removed URL for the discontinued Flexi Vector Primer Design Tool.
2. Removed an expired disclaimer and discontinued products.
3. Updated the cover image.

Printed in USA. Revised 10/21.