We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

Rapid Ligation for the pGEM®-T and pGEM®-T Easy Vector Systems

  • Print
  • Email
  • Download PDF


The pGEM®-T and pGEM®-T Easy Systems are now provided with a new 2X Rapid Ligation Buffer that allows the user to perform ligation reactions in as little as one hour. In this study the performance of the 2X Rapid Ligation Buffer is compared with that of the previously supplied T4 DNA Ligase 10X Buffer in both one-hour and 16-hour ligation reactions. The effects of incubation time, ligation reaction temperature, and insert:vector ratio on cloning efficiencies are also evaluated using the 2X Rapid Ligation Buffer.

Promega Notes 71, 8.

Susan Frackman, and Dan Kephart

Promega Corporation
Publication Date: 1999

Download Article (178 KB)

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.