Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Purifying His-Tagged Proteins from Insect and Mammalian Cells

Natalie Betz
Promega Corporation
Publication Date: 2004


The FastBreak™ Cell Lysis Reagent, 10X and MagneHis™ Protein Purification System can be used to purify His-tagged proteins from insect and mammalian cell lysates and culture media in the presence or absence of serum. This article describes methods for using these reagents with insect and mammalian cells. 

Cell Notes 9, 6–9.

Related Resources