PowerPlex® Fusion 6C System for Use on the Applied Biosystems® Genetic Analyzers Technical Manual
Instructions for Use of Product(s)
DC2705, DC2720, DC2780
Literature # TMD045
The PowerPlex® Fusion 6C System is a 27-locus multiplex that allows co-amplification and fluorescent detection of the 18 autosomal loci in the expanded CODIS core loci (CSF1PO, FGA, TH01, vWA, D1S1656, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433 and D21S11) as well as Amelogenin and DYS391 for gender determination. The Penta D, Penta E, D22S1045, TPOX and SE33 loci are also included to increase discrimination and allow searching of databases that include profiles with these loci. Finally, two rapidly mutating Y-STR loci, DYS570 and DYS576, are included in the multiplex. This extended panel of STR markers is intended to satisfy both CODIS and ESS recommendations.
The PowerPlex® Fusion 6C System is compatible with the Applied Biosystems® 3500 and 3500xL Genetic Analyzers as well as Applied Biosystems® 3130 and 3130xl Genetic Analyzers with Data Collection Software Version 4.0 with the DC v4 6-Dye Module v1 License.
Summary of Changes
The following changes were made to the 11/23 revision of this document:
1. Updated Sections 1 and 11.F.
2. Removed mention of ABI PRISM® Genetic Analyzers.
3. Renumbered figures starting in Section 4.A.
4. Deleted Section 11.B and renumbered subsequent sections.
5. Updated patent and copyright information.
6. Made general text edits.
Revised 11/23.