Wizard® Enviro Total Nucleic Acid Kit Technical Manual
Instructions for Use of Product(s)
A2991
Literature # TM662
Viral or other microbial matter and/or its genetic signature may be present at a low concentration in water samples, making sample concentration a prerequisite for sensitive detection. Concentration of microbial matter can be performed using a variety of methods, such as charged membrane filtration, centrifugal ultrafiltration and flocculation/precipitation using skim milk or polyethylene glycol (PEG)/NaCl. Most of the concentration methods were originally developed to concentrate live matter with the objective of culturing for detection of intact particles, though they have also been used for PCR-based detection. These methods have proven to be inconsistent, labor intensive and time consuming.
To address these issues, we have developed a convenient method to directly capture and concentrate total nucleic acids (TNA) from a large volume of water using PureYield™ columns. The method uses a short protocol that minimizes the need for specialized laboratory equipment.
Summary of Changes
The following changes were made to the 1/23 version of this document:
1. Revised Sections 1, 3, 4 and 7.D.
2. Added new Section 7.C.
3. Updated document font.
Revised 1/23.