Ramos (HiBiT) TCK Bioassay, Propagation Model Technical Manual
Instructions for Use of Product(s)
GA6070, M2472
Literature # TM745
HiBiT TCK Bioassays measure the death of target cells engineered to express a HiBiT fusion protein. HiBiT is an 11 amino acid peptide that binds with high affinity to LgBiT to form NanoBiT® luciferase. When dead or dying cells release the HiBiT fusion protein into the extracellular medium, it binds to the cell impermeable LgBiT, which is provided with the furimazine substrate in a nonlytic, homogeneous detection reagent. In coculture experiments, the luminescent signal will be proportional to target cell death alone, with no signal contribution arising from the death of effector cells.
Ramos (HiBiT) TCK Bioassay, Propagation Model is provided in a cell propagation model (CPM) format, which includes cryopreserved cells that can be thawed, propagated and banked for long-term use. A Ramos (HiBiT) Cell Bank is also available.
When Ramos (HT-HiBiT) target cells are cocultured with appropriate effector cells (e.g., NK cells or cytotoxic T cells) they can be used to specifically monitor the activity of biologics directed against TAA expressed on the Ramos Cells (HT-HiBiT), such as CD19, CD20 or CD22. When Ramos Cells (HT-HiBiT) are used in assays with biologics against TAAs they do not express, the target cells are not lysed and no increase in luminescent signal is observed.
Summary of Changes
The following changes were made to the 5/25 revision of this document:
1. Removed two expired patent statements.
2. Updated text about the label, in Section 3.
Revised 5/25.