Target Cell Killing Bioassays
Target cell killing bioassays offered by Promega provide a simple, sensitive and highly specific method to measure target cell killing induced by a variety of biologic drugs. Upon lysis, the assays generate a bright luminescent signal that can be measured using a standard luminometer.
What Are Target Cell Killing Bioassays?
Targeted killing of tumor or infected cells by effector cells is the primary mechanism of action for many immunotherapy drugs. Thus, it is necessary to demonstrate robust and specific killing of target cells during drug development. Traditional methods used to measure cytotoxicity are not able to distinguish between target and effector cell death. In addition, assays developed to specifically measure the killing of target cells often require radioactive or fluorescent cell labeling, laborious workflows and specialized instruments.
The HiBiT TCK bioassay platform enables highly sensitive and specific measurement of target cell killing induced by a variety of biologic drugs, including monoclonal antibodies (mAbs), bispecific antibodies and CAR-T cells. The assay is simple and homogenous. Upon target cell lysis, the assay produces a bright luminescent signal that can be measured using a standard luminometer.