Raji (HT-HiBiT) TCK Bioassay, Propagation Model Technical Manual
Instructions for Use of Product(s)
M2452, GA6050
Literature # TM718
HiBiT TCK Bioassays measure the death of target cells engineered to express a HiBiT fusion protein. HiBiT is an 11 amino acid peptide that binds with high affinity to LgBiT to form NanoBiT® luciferase. When dead or dying cells release the HiBiT fusion protein into the extracellular medium, it binds to the cell impermeable LgBiT, which is provided with the furimazine substrate in a nonlytic, homogeneous detection reagent. In coculture experiments, the luminescent signal will be proportional to target cell death alone, with no signal contribution arising from the death of effector cells.
Raji (HT-HiBiT) TCK Bioassay, Propagation Model is provided in a cell propagation model (CPM) format, which includes cryopreserved cells that can be thawed, propagated and banked for long-term use. A Raji (HT-HiBiT) Cell Bank is also available.
Summary of Changes
The following changes were made to the 5/25 revision of this document:
1. Removed an expired patent statement.
2. Revised text about the label in Section 3.
Revised 5/25.