NanoBRET™ Target Engagement Intracellular Kinase Assay, Nonbinding Surface Format Technical Manual
Instructions for Use of Product(s)
N2500, N2501, N2520, N2521, N2530, N2540
Literature # TM603
The NanoBRET™ Target Engagement Intracellular Kinase Assay, Nonbinding Surface Format technical manual describes the legacy nonbinding surface (NBS) format of the NanoBRET™ TE Intracellular Kinase Assay. This assay format uses freshly harvested or suspended cells in an NBS assay plate that provides optimal performance for many tracers. Though the NBS workflow is slightly less efficient compared to the new preferred adherent (ADH) format of the assay, it provides quality data using many tracers that are incompatible with conventional polystyrene or tissue-culture treated plates. This assay format is compatible with legacy tracers K-4 and K-5, providing validated assays for 126 kinases. Moreover, the NBS format may be compatible with many of the new NanoBRET™ tracers (K-3, K-8, K-9, K-10 and K-11) with revalidation of the assay. Lastly, this assay format is a strong option to consider for those interested in developing their own NanoBRET™ tracers, particularly if these custom tracers do not perform well in the preferred ADH format.
Summary of Changes
The following change was made to the 7/20 revision of this document:
A url was added to Section 3.A.
Printed in USA. Revised 7/20.