Limited Functionality Warning

We are currently updating our network. During this time, certain functionality may be unavailable, including online orders. We apologize for any inconvenience this may cause you.

Please contact Customer Service with any questions or comments.
Phone: (608) 274-4330
Toll-Free Phone: (800) 356-9526
Hours: 7am – 6pm, CST, Monday-Friday

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Nano-Glo® HiBiT Extracellular Detection System Technical Manual

Instructions for Use of Product(s)
N2420, N2421, N2422

Literature # TM523

The Nano-Glo® HiBiT Extracellular Detection System sensitively quantifies HiBiT-tagged proteins expressed in the plasma membrane or secreted into the extracellular medium using a simple add-mix-read assay protocol. HiBiT is an 11-amino-acid peptide tag that is fused to the N or C terminus of the protein of interest or inserted into an accessible location within the protein structure. The amount of a HiBiT-tagged protein present on the cell surface or secreted is determined by adding a nonlytic detection reagent containing the substrate furimazine and Large BiT (LgBiT), the large subunit used in NanoLuc Binary Technology (NanoBiT®). HiBiT binds tightly to LgBiT (KD = 0.7nM), forming a complex that generates a bright, luminescent enzyme. The amount of luminescence generated after adding the Nano-Glo® HiBiT Extracellular Reagent is proportional to the amount of HiBiT-tagged protein accessible to the extracellular medium over seven orders of magnitude.

Printed in the USA 8/17.