ICOS Blockade Bioassay Technical Manual
Instructions for Use of Product(s)
JA6001, JA6005, JA7001, JA7005
Literature # TM629
The ICOS Blockade Bioassay is a bioluminescent cell-based assay that can be used to measure the potency and stability of antibodies and other biologics that block ICOS/ICOSL. The assay consists of two genetically engineered cell lines, ICOS Effector Cells and ICOSL aAPC/CHO-K1 Cells. These cells are provided in thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation.
When the two cell types are cocultured, the ICOSL aAPC/CHO-K1 Cells activate TCR/CD3 and ICOS on the ICOS Effector Cells to induce maximum promoter-mediated luminescence. Adding a biologic that blocks ICOS/ICOSL inhibits costimulation by ICOS and results in decreased promoter-mediated luminescence. The bioluminescent signal is quantified using the Bio-Glo-NL™ Luciferase Assay System (Cat.# J3081), and a standard luminometer such as the GloMax® Discover System.
This Technical Manual is relevant for Cat.# JA6001 and JA6005; JA7001 and JA7005 (Korea).
Summary of Changes
The following change was made to the 5/25 revision of this document:
1. Revised text about the label, in Section 3.
Revised 5/25.