GoTaq® Enviro Wastewater SARS-CoV-2 Systems Technical Manual
Instructions for Use of Product(s)
AM2100, AM2110, AM2120, AM2130
Literature # TM661
The GoTaq® Enviro Wastewater SARS-CoV-2 Systems for wastewater samples are four-dye, three-target hydrolysis probe-based reverse transcription (RT)-qPCR kits that amplify SARS-CoV-2 genetic signatures. The systems include primer/probe sets prescribed by the U.S. Centers for Disease Control (CDC) that target the N1 or N2 regions of the SARS-CoV-2 nucleocapsid (N) gene, or a primer/probe set targeting the envelope (E) gene. The systems are designed for a one-step RT-qPCR. As an internal process control and to allow for normalization, the systems include primers to detect Pepper Mild Mottle Virus (PMMoV), an RNA virus commonly found in wastewater, and an internal amplification control (IAC).
Summary of Changes
The following changes were made to the 4/24 revision of this document:
1. Added Section 8.C, Troubleshooting.
2. Made minor text edits.
Revised 4/24.