GoTaq® Enviro Wastewater RSV, Flu, SC2 System Technical Manual
Instructions for Use of Product(s)
AM2180
Literature # TM764
The GoTaq® Enviro Wastewater RSV, Flu, SC2 System is a four-dye four-target hydrolysis probe-based assay designed to quantify Respiratory Syncytial Virus A and B subtypes (RSV), Influenza A and B subtypes (Flu), and SARS-CoV-2 (SC2) RNA from test samples, such as wastewater, in a single-step RT-qPCR amplification. The system includes primer/probe sets that target Influenza A (FluA), Influenza B (FluB) and the 3´ untranslated region of the SARS-CoV-2 nucleocapsid (N) gene. The primer/probe set also detects RSV-A and RSV-B, which is based on Nadeau et al. 2024. In addition, the primer/probe set detects Pepper Mild Mottle Virus (PMMoV), an RNA virus commonly found in wastewater, as a control. The GoTaq® Enviro Master Mix uses proprietary enzymes and formulations that tolerate reverse transcriptase and PCR inhibitors, such as humic acids, that can be present in nucleic acid samples purified from wastewater.
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