GoTaq® Enviro qPCR System Technical Manual
Instructions for Use of Product(s)
AM2000, AM2001
Literature # TM658
The GoTaq® Enviro qPCR System is optimized for quantitative PCR assays using a hydrolysis probe for real-time amplicon detection. The system amplifies DNA targets from environmental samples that may contain PCR inhibitors, such as humic acid, tannic acid and other compounds. The GoTaq® Enviro qPCR System includes GoTaq® Enviro qPCR Master Mix, a ready-to-use, stabilized 2X formulation that includes all components for qPCR, including GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer.
The GoTaq® Enviro Master Mix provides resistance to a wide range of PCR inhibitors commonly found in environmental samples. This formulation uses antibody-mediated hot-start chemistry, allowing reaction setup to be performed at room temperature. The master mix also employs rapid hot-start activation and processive enzymes, making it compatible with both standard and fast instrument cycling programs.
Summary of Changes
The following changes were made to the 3/24 revision of this document:
1. Troubleshooting, Section 5.C, was added.
2. Previous Sections 5 and 6 were combined with the Appendix.
Revised 3/24.