GITR Bioassay, Propagation Model, Technical Manual
Instructions for Use of Product(s)
J2272
Literature # TM583
The GITR Bioassay, Propagation Model, is a bioluminescent cell-based assay that overcomes the limitations of existing assays and can be used to measure the potency and stability of ligands or agonist antibodies that can bind and activate GITR. The assay consists of a genetically engineered Jurkat T cell line that expresses human GITR and a luciferase reporter driven by a response element that can respond to GITR ligand/agonist antibody stimulation. The GITR Effector Cells are provided in Cell Propagation Model (CPM) format, as cryopreserved cells that can be thawed, propagated and banked for long-term use.
Summary of Changes
The following changes were made to the 5/25 revision of this document:
1. Updated Lumit to a registered trademark.
2. Revised text about the label in Section 3.
3. Removed an expired patent statement.
4. Updated a third party trademark.
Revised 5/25.