GDP-Glo™ Glycosyltransferase Assay Technical Manual
Instructions for Use of Product(s)
VA1090, VA1091, VA1092
Literature # TM505
The GDP-Glo™ Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use GDP-sugars as donor substrates and release GDP as a product. Once the glycosyltransferase reaction is complete, an equal volume of GDP Detection Reagent is added to simultaneously convert the GDP product to ATP and generate light in a luciferase reaction. The light generated is detected using a luminometer. Luminescence can be correlated to GDP concentration by using a GDP standard curve. The light output is proportional to the concentration of GDP from low nM to 25μM.
Printed in USA 10/17.