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GDP-Glo™ Glycosyltransferase Assay

VA1090_GDP-Glo-Glycosyltransferase-Assay

Universal Glycosyltransferase Activity Assay

  • Easy, "add-incubate-read" protocol
  • Detects any glycosyltransferase that uses GDP-sugar as a substrate
  • Ideal for low-activity glycosyltransferases

Size

Add a GDP-sugar substrate

Catalog number selected: VA1090

$ 318.00
Your price:
Add to Cart
This product is discontinued
GDP-Glo™ Glycosyltransferase Assay
200 assays
$ 318.00
Your price: Log in
Change Configuration
36565602-GDP-Glo-UMP-CMP-Glo-3d-Illustration

A Simple, Bioluminescent Assay for Glycosyltransferase Activity

The GDP-Glo™ Glycosyltransferase Assay is a homogeneous, single-reagent-addition method to rapidly detect GDP formation in glycosyltransferase reactions. After the glycosyltransferase reaction, an equal volume of GDP Detection Reagent is added to simultaneously convert the GDP product to ATP and generate light in a luciferase reaction. The light generated is detected using a luminometer, and the light output is proportional to the concentration of GDP from low nM to 25μM. Luminescence can be correlated to GDP concentration by using a GDP standard curve.

Add-Mix-Read for Results

Start with purified enzymes, sugar donors and acceptor molecules, and you can detect the glycosyltransferase reaction product GDP. Just add the GDP Detection Reagent and measure luminescence.

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Works with Most Glycosyltransferases

The GDP-Glo™ Assay can be used to measure the activity of just about any glycosyltransferase that uses GDP-sugar as a substrate. Here, we looked at Fucosyltransferases involved in antigen display on immune cells.

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Detects Low Nanomolar Amounts of GDP; Ideal for Low-Activity Glycosyltransferases

The sensitivity of the GDP-Glo™ Glycosyltransferase Assay means it can detect low GDP concentrations with high dynamic range. Therefore, whether assaying for a low-activity glycosyltransferase whose sugar transfer rate is low or using a low concentration of enzyme that produces a small amount of GDP, you can obtain a high signal-to-background ratio using the assay.

The data below show the linearity and sensitivity of the GDP-Glo™ Glycosyltransferase Assay. Here, a GDP standard curve was prepared over the indicated concentration range in 25µl of 1X glycosyltransferase reaction buffer in a solid white 96-well plate. GDP-Glo™ Glycosyltransferase Assay was performed using 25µl of GDP Detection Reagent at room temperature. Luminescence was recorded using a GloMax® luminometer. Values represent the mean of four replicates. The table shows luminescence measured 1 hour after adding the GDP Detection Reagent, and signal-to-background ratio calculated for each concentration of the standard curve.

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Luminescent Signal is Stable Over Several Hours

The GDP-Glo™ Assay  generates a highly stable luminescent signal that is stable for more than 3 hours, making it convenient to batch-process multiple assay plates.

The data here show that the signal obtained with the GDP-Glo™ Assay is stable for several hours. To determine signal stability, luminescence was recorded at 1 and 3 hours after adding detection reagent. The signal-to-background ratio did not change over the time measured (data not shown). RLU = relative light units.

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Specifications

You are viewing: VA1090 Change Configuration

What's in the box?

Item Part # Size

ATP Detection Substrate

V363C 1 × 1 vial

Nucleotide Detection Buffer

V696A 1 × 5ml

Enzyme Dilution Buffer

V773A 1 × 1.5ml

GDP, 10mM

VA111A 1 × 100μl

GDP-Glo™ Enzyme

VA112A 1 × 20μl

SDS

Choose language:

Download SDSPDF (196 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Specifications

You are viewing: VA1091 Change Configuration

What's in the box?

Item Part # Size

ATP Detection Substrate

V363A 1 × 1 vial

Nucleotide Detection Buffer

V696B 1 × 10ml

Enzyme Dilution Buffer

V773A 2 × 1.5ml

GDP, 10mM

VA111A 1 × 100μl

GDP-Glo™ Enzyme

VA112B 1 × 40μl

SDS

Choose language:

Download SDSPDF (196 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Specifications

You are viewing: VA1092 Change Configuration

What's in the box?

Item Part # Size

ATP Detection Substrate

V363B 1 × 1 vial

Nucleotide Detection Buffer

V696C 1 × 100ml

Enzyme Dilution Buffer

V773B 1 × 15ml

GDP, 10mM

VA111A 1 × 100μl

GDP-Glo™ Enzyme

VA112C 1 × 200μl

SDS

Choose language:

Download SDSPDF (196 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Specifications

You are viewing: VA1093 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

ATP Detection Substrate

V363C 1 × 1 vial

Nucleotide Detection Buffer

V696A 1 × 5ml

Enzyme Dilution Buffer

V773A 1 × 1.5ml

GDP, 10mM

VA111A 1 × 100μl

GDP-Glo™ Enzyme

VA112A 1 × 20μl

GDP-Fucose

VA109A 1 × 50μl50mM View Product

SDS

Choose language:

Download SDSPDF (219 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Specifications

You are viewing: VA1094 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

ATP Detection Substrate

V363A 1 × 1 vial

Nucleotide Detection Buffer

V696B 1 × 10ml

Enzyme Dilution Buffer

V773A 2 × 1.5ml

GDP, 10mM

VA111A 1 × 100μl

GDP-Glo™ Enzyme

VA112B 1 × 40μl

GDP-Fucose

VA109A 1 × 50μl50mM View Product

SDS

Choose language:

Download SDSPDF (220 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Specifications

You are viewing: VA1095 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

ATP Detection Substrate

V363C 1 × 1 vial

Nucleotide Detection Buffer

V696A 1 × 5ml

Enzyme Dilution Buffer

V773A 1 × 1.5ml

GDP, 10mM

VA111A 1 × 100μl

GDP-Glo™ Enzyme

VA112A 1 × 20μl

GDP-Mannose

VA110A 1 × 50μl100mM View Product

SDS

Choose language:

Download SDSPDF (220 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

Specifications

You are viewing: VA1096 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

ATP Detection Substrate

V363A 1 × 1 vial

Nucleotide Detection Buffer

V696B 1 × 10ml

Enzyme Dilution Buffer

V773A 2 × 1.5ml

GDP, 10mM

VA111A 1 × 100μl

GDP-Glo™ Enzyme

VA112B 1 × 40μl

GDP-Mannose

VA110A 1 × 50μl100mM View Product

SDS

Choose language:

Download SDSPDF (219 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

European Pat. No. 1131441 and Japanese Pat. No. 4520084.

U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.

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