CD40 Bioassay, Propagation Model
Instructions for Use of Product(s)
J2132
Literature # TM552
The CD40 Bioassay, Propagation Model (Cat.# J2132), is a bioluminescent cell-based assay that overcomes the limitations of existing assays and can be used to measure the potency and stability of ligands or agonist antibodies and other biologics that can bind and activate CD40. The assay consists of CD40 Effector Cells that express human CD40. CD40 Effector Cells are also genetically engineered with a luciferase reporter driven by a response element that can respond to CD40 ligand/agonist antibody stimulation. The CD40 Effector Cells are provided in Cell Propagation Model (CPM) format, which includes cryopreserved cells that can be thawed, propagated and banked for long-term use.
Based on the antibody properties to be tested, the CD40 Bioassay can be conducted in either single-cell systems or with FcγRIIb CHO-K1 Cells (Cat.# J2232) to determine the agonist antibodies that can activate CD40 in a FcγR-dependent manner. Induction of the CD40 Effector Cells with CD40 ligand or an agonist CD40 antibody results in response element-mediated luminescence. The bioluminescent signal is quantified using the Bio-Glo™ Luciferase Assay System and a standard luminometer such as the GloMax® Discover System.
Summary of Changes
The following changes were made to the 6/25 revision of this document:
1. Removed an expired patent statement and updated another patent statement.
2. Updated text about the label in Section 3.
3. Made miscellaneous text edits.
Revised 6/25.