ReliaPrep™ FFPE Total RNA Miniprep System Technical Manual

Instructions for Use of Product(s)
Z1001, Z1002
Literature # TM353

The ReliaPrep™ FFPE Total RNA Miniprep System optimizes lysis conditions to reverse RNA modifications caused by formalin fixation without the need for overnight digestion. This system also uses a deparaffinization method that does not rely on xylene.

Summary of Changes
The following changes were made to the 12/15 revision of this document:
1. Blue Dye is now included in the ReliaPrep™ FFPE Total RNA Miniprep System.
2. A step was added for preparing the Lysis Buffer with the Blue Dye.
3. The lysis step in the method now includes a 1 hour incubation at 80°C instead of 15 minutes.
4. An optional overnight storage step was added after the 80°C incubation of the lysis step.

Printed in USA. Revised 12/15.