MTase-Glo™ Methyltransferase Assay Technical Manual

Instructions for Use of Product(s)
V7601, V7602

Literature # TM453

The MTase-Glo™ Assay is a bioluminescence-based assay that can be used to monitor the activities of methyltransferasesmethyltransferases (MTases) and their modulation by small molecules in high-throughput screening applications. The assay monitors formation of the reaction product S-adenosyl homocysteine (SAH) and can detect changes in activity of a broad range of methyltransferases, including DNA, protein, RNA and small molecule methyltransferases. The MTase-Glo™ Assay can be used for all classes of protein methyltransferases (lysine and arginine) and with different types of substrates (peptides, large proteins and even nucleosomes) to determine the specificity of these enzymes and their substrate requirements.

Summary of Changes
The following changes were made to the 3/24 revision of this document:
1. Corrected the figure legend of Figure 9. to change 0.5ng to 0.5µg.
2. Updated font and cover image.
3. Made minor text edits.

Revised 3/24.