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HDAC-Glo™ 2 Assay

Instructions for Use of Product(s)

Literature # TM406

The HDAC-Glo™ 2 Assay is a single-reagent addition, homogeneous, luminescence assay that selectively measures the relative activity of histone deacetylase (HDAC) 2 enzyme from cells, extracts or recombinant sources. The assay uses an isoenzyme-selective, acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC 2 activity. Deacetylation of the peptide substrate is measured using a coupled enzymatic system in which a protease in the Developer Reagent cleaves the peptide from aminoluciferin, which is quantified in a reaction using Ultra-Glo™ Recombinant Luciferase.

Summary of Change

The following change was made to the 11/17 revision of this document:
Removed any mention of discontinued products.

Revised 11/17.

Experienced User Protocols