Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

PureYield™ Plasmid Midiprep System Technical Manual

Instructions for Use of Product(s)
A2492, A2495, A2496

Literature # TM253

The PureYield™ Plasmid Midiprep System is designed to isolate high-quality plasmid DNA for use in eukaryotic transfection and in vitro expression experiments. The system provides a rapid method for purification of 100–200µg of plasmid DNA from transformed E. coli cultures using a newly developed silica-membrane column. Plasmid DNA can be purified in less than 30 minutes, greatly reducing the time spent on purification compared to silica-resin or other membrane column methods.

Summary of Change
The following change was made to the 12/17 revision of this document:
1. Supplemental protocol on DNA purification by vacuum only was removed.

Printed in USA. Revised 12/17.

Experienced User Protocols