PureYield™ Plasmid Midiprep System Technical Manual
Literature # TM253
The PureYield™ Plasmid Midiprep System is designed to isolate high-quality plasmid DNA for use in eukaryotic transfection and in vitro expression experiments. The system provides a rapid method for purification of 100–200µg of plasmid DNA from transformed E. coli cultures using a newly developed silica-membrane column. Plasmid DNA can be purified in less than 30 minutes, greatly reducing the time spent on purification compared to silica-resin or other membrane column methods.
Summary of Change
The following change was made to the 12/17 revision of this document:
1. Supplemental protocol on DNA purification by vacuum only was removed.
Printed in USA. Revised 12/17.