Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

PureYield™ Plasmid Midiprep System Technical Manual

Instructions for Use of Product(s)
A2492, A2495, A2496

Literature # TM253

The PureYield™ Plasmid Midiprep System is designed to isolate high-quality plasmid DNA for use in eukaryotic transfection and in vitro expression experiments. The system provides a rapid method for purification of 100–200µg of plasmid DNA from transformed E. coli cultures using a newly developed silica-membrane column. Plasmid DNA can be purified in less than 30 minutes, greatly reducing the time spent on purification compared to silica-resin or other membrane column methods.

Summary of Change
The following change was made to the 12/17 revision of this document:
1. Supplemental protocol on DNA purification by vacuum only was removed.

Printed in USA. Revised 12/17.

Experienced User Protocols