MagneSil® Total RNA mini-Isolation System Protocol
Instructions for Use of Product(s)
Literature # TB328
The MagneSil® Total RNA mini-Isolation System provides a high-throughput 96-well format for fast, simple purification of intact, purified total RNA from small amounts of cell culture (≤1 × 105 tissue culture cells), tissue (≤2mg tissue lysate in 100μl), or freshly isolated whole blood (≤20μl). The protocol enables manual purification using a multichannel pipettor and plate shaker or high-throughput automated purification on a variety of liquid-handling workstations. Isolation of total RNA in a 384-well format from cell culture (≤1 × 103 cells) and freshly isolated whole blood (≤5μl) also may be performed. Total RNA purification is achieved without the need for vacuum filtration, centrifugation or precipitation. The 96-well total RNA isolation procedure takes about 30 minutes to complete on a liquid-handlingworkstation.
Printed in USA. Revised 12/10.