We believe this site might serve you best:

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Calpain-Glo™ Protease Assay Technical Bulletin

Instructions for Use of Product(s)
G8501, G8502
Literature # TB344

The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain I (μ) and II (m) activities. The Calpain-Glo™ Protease Assay provides a succinyl proluminescent calpain substrate, Suc-LLVY-aminoluciferin, in a buffer system optimized for calpain activity and luciferase activity. Adding the single Calpain-Glo™ Reagent to test samples in an “add-mix-measure” format results in calpain cleavage of the substrate and generation of a “glow-type” luminescent signal produced by the luciferase reaction. In this homogeneous, coupled-enzyme format, the signal is proportional to the amount of calpain activity present. The calpain and luciferase enzyme activities reach a steady-state so that the luminescent signal peaks rapidly and achieves maximum sensitivity in 5–15 minutes. The homogeneous Calpain-Glo™ Assay is designed for use with multiwell plate formats, making it ideal for automated high-throughput screening (HTS) of calpain activity and inhibition.

Summary of Changes
The following changes were made to the 12/15 revision of this document:
1. Patent and disclaimer statements were updated.
2. The document design was updated.

Printed in USA. Revised 12/15.

Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom