Wizard® MagneSil® Sequencing Reaction Clean-Up System Protocol
Literature # TB287
The MagneSil® Paramagnetic Particles allow purification of nucleic acids such as plasmid DNA and PCR fragments. This technology also can be applied to the high-throughput purification of BigDye® terminator DNA sequencing reactions prior to automated sequence analysis. Procedures exist for several robotic workstations using the standard 96-well amplification plate format, offering the distinct advantage of a hands-off protocol. Sequence quality is similar to or better than standard manual methods. No user intervention is required from the time the 96-well amplification plate is put on the robot deck until the samples are loaded onto the DNA sequencer.
Printed in USA. Revised 12/10.