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MagneSil® GREEN

MagneSil GREEN 100ml
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High-Throughput Purification of DNA from Sequencing Reactions

  • Validated, walkaway method
  • Purified products suitable for fluorescent sequencing

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Catalog number selected: A8231

$ 314.00
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MagneSil® GREEN
100ml
$ 314.00
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High-throughput, automated purification of sequencing reactions, including BigDye® Terminator reactions, is performed using MagneSil® GREEN Paramagnetic Particles with standard, nonskirted 96-well amplification plates. No user intervention is required from the time the plates are placed on the instrument until the samples are ready for loading onto the fluorescent DNA sequencer. Purified products are approved for fluorescent sequencing reactions (Phred* 20 quality scores ≥650 bases). Protocols are available for automated instruments from Beckman Coulter and Tecan.

The system relies upon the MagnaBot® II for magnetic separation. The Plate Clamp 96 and Plate Stand are recommended for automated use because they ensure PCR plates are uniformly flat for liquid transfer on a robotic instrument.

*A Phred score is a widely recognized method to measure the quality of DNA sequences. Phred is a base-calling program for DNA sequence traces available from Codoncode Corporation.

Specifications

What's in the box?

Item Part # Size

MagneSil® GREEN

A8231 1 × 100ml

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Patents and Disclaimers

U.S. Pat. Nos. 6,284,470 and 7,078,224 and other patents and patents pending.

Resources

Citations

  • Automated purification of dye terminator sequencing reactions: An approach to high-throughput capillary electrophoresis sequencing of large templates.


    --
    2003 JALA
  • See all citations

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