High-throughput, automated purification of sequencing reactions, including BigDye® Terminator reactions, is performed using MagneSil® GREEN Paramagnetic Particles with standard, nonskirted 96-well amplification plates. No user intervention is required from the time the plates are placed on the instrument until the samples are ready for loading onto the fluorescent DNA sequencer. Purified products are approved for fluorescent sequencing reactions (Phred* 20 quality scores ≥650 bases). Protocols are available for automated instruments from Beckman Coulter and Tecan.
The system relies upon the MagnaBot® II for magnetic separation. The Plate Clamp 96 and Plate Stand are recommended for automated use because they ensure PCR plates are uniformly flat for liquid transfer on a robotic instrument.
*A Phred score is a widely recognized method to measure the quality of DNA sequences. Phred is a base-calling program for DNA sequence traces available from Codoncode Corporation.