Instructions for Use of Product(s)
Literature # TB166
The RiboMAX™ Large Scale RNA Production Systems—SP6 and T7 (Cat.#P1280, P1300) are designed for applications that require milligram quantities of biologically active RNA transcripts. In vitro transcription reactions that produce large amounts of RNA are potentially valuable for in vitro translation; for synthesis of tRNA, rRNA, other small functional RNAs, RNA virus genomes and ribozymes; for production of substrates for studies of RNA splicing, RNA secondary structure, antisense RNA and RNA-protein interactions; as well as oocyte injections and mammalian transfection assays.
Summary of Change
The following change was made to the 5/17 revision of this document:
Corrected final concentration of 5X MOPS buffer in RNA sample buffer recipe (Section 6).
Printed in USA. Revised 5/17
Experienced User Protocols