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DeadEnd™ Colorimetric TUNEL System Technical Bulletin

Instructions for Use of Product(s)
G7130, G7360

Literature # TB199

The DeadEnd™ Colorimetric TUNEL System end-labels the fragmented DNA of apoptotic cells using a modified TUNEL (TdT-mediated dUTP Nick-End Labeling) assay. Biotinylated nucleotide is incorporated at the 3′-OH DNA ends using the enzyme Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT). Horseradish-peroxidase-labeled streptavidin (Streptavidin HRP) is then bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and the stable chromogen, diaminobenzidine (DAB). Using this procedure, apoptotic nuclei are stained dark brown.

The following changes were made to the 1/19 revision of this document:
1. Product description has been updated to include information about how the detection is performed with a light microscope.
2. Product components list has been updated to reflect new names and sizes.
a. Hydrogen Peroxide 20X is no longer included in this system and references in the protocol have been removed.
3. Reagent Preparation information has been added to Section 3.A.
4. New Related Products have been added and discontinued products have been removed.

Printed in USA. Revised 1/19.

Experienced User Protocols