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CytoTox 96® Non-Radioactive Cytotoxicity Assay Technical Bulletin

Instructions for Use of Product(s)

Literature # TB163

The CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to 51Cr release cytotoxicity assays. The assay measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis. Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay, which results in conversion of a tetrazolium salt (INT) into a red formazan product. The amount of color formed is proportional to the number of lysed cells. Visible wavelength absorbance data are collected using a standard 96-well plate reader.

Summary of Changes
The following change was made to the 7/16 revision of this document:
1. The name of the Vehicle-Only Cells Control was clarified in the Maximum LDH Release Control listed in Section 4.A.

Printed in USA. Revised 7/16.