pF3A WG (BYDV) Flexi® Vector
Instructions for Use of Product(s)
Literature # 9PIL567
The pF3A WG (BYDV) Flexi® Vector is designed for use with the Flexi® System, Entry/Transfer (Cat.# C8640), and the Flexi® System, Transfer (Cat.# C8820). The vector contains translation enhancer (TE) sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, that facilitate in vitro expression of protein in wheat germ extract. T7 and SP6 RNA polymerase promoters allow production of RNA for subsequent translation applications. Linear DNA templates for in vitro RNA transcription can be produced by cutting at one of the restriction enzyme sites located downstream of the 3´ BYDV TE region. The vector also contains the lethal barnase gene for positive selection of the insert, an ampicillin-resistance gene for selection of the plasmid in E. coli and unique SgfI and PmeI sites, which allow easy insertion or transfer of the sequence of interest. Inserts containing a protein-coding region can easily be transferred from the pF3A WG (BYDV) Flexi® Vector to other Flexi® Vectors with different expression options. For more information, see the Flexi® Vector Systems Technical Manual #TM254 or the Wheat Germ Extract Plus Technical Manual #TM066.