AMV Reverse Transcriptase Protocol
Literature # 9PIM510
AMV Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity, but the AMV RT preparation is devoid of other contaminating DNases and RNases.
Printed in USA. Revised 10/16.