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AMV Reverse Transcriptase

M1701

Efficient Reverse Transcription of Templates with High Secondary Structure

  • DNA polymerization using template DNA, RNA or RNA:DNA hybrids
  • Requires a primer as well as Magnesium or Manganese
  • Stability at higher reaction temperatures (37–58°C)

Size

Catalog number selected: M5101

$ 103.00 Your price: Log In

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AMV Reverse Transcriptase
300u
$ 103.00
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Reverse Transcriptase with Stability at Higher Temperatures

Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.

Features
  • High Concentration: Cat.# M9004 contains 600 units of AMV RT at 20–25u/μl.
  • 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 50mM MgCl2, 2.5mM spermidine, 50mM DTT.
  • Temperature Stability: AMV RT is the preferred reverse transcriptase for templates with high secondary structure due to its stability at higher reaction temperatures (37–58°C).
Applications
  • First- and second-strand synthesis of cDNA.
  • Primer extensions and RNA sequencing.
  • RT-PCR. Up to 10μl of an RT reaction containing AMV RT and the supplied AMV RT Reaction Buffer can be added to a 50μl PCR amplification reaction that uses Taq DNA polymerase. If GoTaq® DNA Polymerase or PCR Master Mix are used, up to 25μl of an RT reaction can be added per 50μl PCR.
Further Reading
  1. Kacian, D.L. (1977) Meth. Virol. 6, 143.
  2. Mierendorf, R.C. and Pfeffer, D. (1987) Meth. Enzymol. 152, 563–6.

Protocols

Specifications

You are viewing: 300u Change Configuration

What's in the box?

Item Part # Size

AMV Reverse Transcriptase

M510A 1 x 300u
AMV RT 5X Buffer M515A 1 x 500μl

SDS

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Certificate of Analysis

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Store at –20°C.

Specifications

You are viewing: 1,000u Change Configuration

What's in the box?

Item Part # Size

AMV Reverse Transcriptase

M510F 1 x 1,000u
AMV RT 5X Buffer M515A 3 x 500μl

SDS

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Certificate of Analysis

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Store at –20°C.

Specifications

You are viewing: 600u Change Configuration

What's in the box?

Item Part # Size
AMV RT 5X Buffer M515A 1 x 500μl

AMV Reverse Transcriptase (HC)

M900A 1 x 600u

SDS

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Certificate of Analysis

Search for Specific Certificate:

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Store at –20°C.

Resources

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RNA Analysis Workflow

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