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pGL4.34[luc2P/SRF-RE/Hygro] Vector Protocol

Instructions for Use of Product(s)

Literature # 9PIE135

The pGL4.34[luc2P/SRF-RE/Hygro] Vector contains a Serum Response Factor Response Element (SRF-RE) that drives the transcription of the luciferase reporter gene luc2P in response to activation of Serum Response Factor through multiple signaling pathways including activation of RhoA GTPase. luc2P is a synthetically-derived luciferase sequence with humanized codon optimization. The luc2P gene also contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly to induction than the protein encoded by the luc2 gene. The vector backbone contains an ampicillin resistance gene to allow for selection in E. coli and the mammalian-selectable marker for hygromycin resistance.

Printed in USA. Revised 10/16.

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