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pFN11A (BIND) Flexi® Vector Product Information

Instructions for Use of Product(s)

Literature # 9PIC934

The pFN11A (BIND) Flexi® Vector is designed for use with the CheckMate™/Flexi® Vector Mammalian Two- Hybrid System (Cat.# C9360), the Flexi® System, Entry/Transfer (Cat.# C8640) and the Flexi® System, Transfer (Cat.# C8820). The pFN11A (BIND) Flexi® Vector contains the yeast Gal4 DNA-binding domain upstream and in-frame with the cloning site. The vector also expresses the Renilla reniformis luciferase gene under the control of the SV40 promoter, allowing normalization for differences in transfection efficiency. This vector can be used to test putative transcriptional activation domains for protein sequences of interest when co-transfected with the pGL4.31[luc2P/Gal4 UAS/Hygro Vector(Cat.# C9351). In common with other Flexi® Vectors, the pFN11A (BIND) Flexi® Vector contains a CMV immediate early enhancer/promoter region plus a chimeric intron for mammalian expression, a T7 promoter for in vitro expression of the protein-coding region, a barnase gene for positive selection of the insert, an ampicillin resistance gene for selection of the plasmid, and unique Sgf I and Pme I sites that allow easy insertion and transfer of the insert of interest. Do not use the pFN11A (BIND) Flexi® Vector without an insert as a negative control because the presence of the barnase gene decreases the viability of the transfected cells. The pFN11A (BIND) Flexi® Vector containing a cloned protein-coding region can be used to transfer the insert to other Flexi® Vectors with different expression options using the Flexi® Systems. For more information, see the CheckMate™/Flexi ® Vector Mammalian Two-Hybrid System Technical Manual #TM283, and the Flexi ® Vector Systems Technical Manual #TM254.

Printed in USA. Revised 9/16