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For ordering information on the products discussed here, please visit our Restriction Enzymes product listing. 

The 10X Reaction Buffer supplied with each restriction enzyme is optimized to give 100% activity. In many cases, good activity is also obtained using one of the 4-CORE® 10X Buffers (Cat.# R9921). This reference table may be used to select the best buffer for digestions with multiple restriction enzymes. Enzyme activity is expressed as a percent of the activity obtained with the optimized buffer for each enzyme.

Promega Enzyme Buffer Supplied with Enzyme 4 CORE® Buffers MULTI-CORE™ Buffer Enzyme Assay Temperature
    A B C D    
AgeI K 25–50% 25–50% 25–50% 50–75% 100% 37°C
AluI B 75–100% 100% 75–100% 10–25% 10–25% 37°C
ApaI A 100% 50–75% 50–75% <10% 75–100% 37°C
BamHI E 75–100% ** 75–100% 75–100% 50–75% 75–100% 37°C
BclI C 10–25% 75–100% 100% 50–75% 10–25% 50°C
BglI D 10–25% 25–% 75–100% 100% 100% 37°C
BglII D 25–50% 75–100% 75–100% 100% <10% 37°C
CfoI B 75–100% 100% 75–100% 25–50% 100% 37°C
ClaI C 75–100% 75–100% 100% 75–100% 100% 37°C
DdeI D 25–50% 25–50% 50–75% 100% 25–50% 37°C
DpnI B 50–75% 100% 75–100% 50–75% 100% 37°C
EcoRI H 25–50% 50–75% 50–75% 50–75% 100%** 37°C
EcoRV D 10–25% 25–50% 50–75% 100% 100% 37°C
HaeIII C 75–100% 75–100% 100% 50–75% 100% 37°C
HhaI C 50–75% 75–100% 100% 50–75% 75–100% 37°C
HindIII E 25–50% 100% 75–100% 10–25% 50–75% 37°C
HinfI B 50–75% 100% 75–100% 75–100% 50–75% 37°C
HpaII A 100% 50–75% 50–75% 10–25% 100% 37°C
I-PpoI I-PpoI 10–25% 25–50% 25–50% 25–50% 37°C
KpnI J 100%** 25–50% 25–50% <10% 75–100% 37°C
MboI C 10–25% 75–100% 100% 50–75% <10% 37°C
MluI D 10–25% 25–50% 50–75% 100% 10–25% 37°C
MspI B 75–100% 100% 75–100% 25–50% 25–50% 37°C
NcoI D 50–75% 75–100% 75–100% 100% 75–100% 37°C
NdeI D <10% <10% 25–50% 100% 25–50% 37°C
NheI B 75–100% 100% 75–100% 10–25% 100% 37°C
NotI D <10% 10–25% 25–50% 100% 25–50% 37°C
PstI H 10–25% 50–75% 50–75% 50–75% 25–50% 37°C
PvuI D 10–25% 25–50% 50–75% 100% <10% 37°C
RsaI C 75–100% 75–100% 100% <10% <10% 37°C
SacI J 75–100% 25–50% 25–50% <10% 100% 37°C
SacII C 100%* 50–75% 100% 50–75% <10% 37°C
SalI D <10% 10–25% 25–50% 100% <10% 37°C
ScaI K <10% 100%** 50–75% 75–100% 10–25% 37°C
SgfI C 25–50% 25–50% 100% <10% <10% 37°C
SmaI J <10% <10% 10% <10% 100% 25°C
SpeI B 75–100% 100% 75–100% 75–100% 100% 37°C
SphI K 75–100% 75–100%** 100% 75–100% 10–25% 37°C
TaqI E 10–25% 25–50% 50–75% 50–75% 100% 65°C
XbaI D 50–75% 75–100% 75–100% 100% 100% 37°C
XhoI D 25–50% 75–100% 75–100% 100% 10–25% 37°C