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My cells are dying! How can I tell if it is apoptosis or necrosis?

Understanding Mechanism of Action: Cytotoxicity

Multiplexing assays gathers more than one set of data from the same sample. The CellTox™ Green Cytotoxicity Assay can be multiplexed with other spectrally distinct measures of cell health to provide more information about why cells are dying. The CellTiter-Fluor™ Cell Viability Assay measures the activity of a conserved, constitutive protease within live cells and serves as a marker of cell viability. Caspase-3/7 activation is frequently used as a positive marker for apoptosis. The Caspase-Glo® 3/7 Assay provides a sensitive means of monitoring caspase activation.

Understanding Mechanism of Action: Cell Proliferation

The key benefit of multiplexing is gaining a better understanding of the event you are measuring in the context of another parameter, minimizing faulty interpretation of data or ambiguity from data sets. Multiplexing can show that an apparent reduction in cell viability is actually from a nonproliferative effect, not cytotoxicity.

Test Your Knowledge With These Three Experiments

SAHA
Camptothecin
FK866
13166MA

Cells were treated with SAHA at various concentrations for 48 hours. The mechanism of action was assessed using a multiplex of CellTox™ Green, CellTiter-Fluor™ and Caspase-Glo® Assays.

Interpret The Data

Based on the data, what can be determined about this compound's mechanism of action?

(Select more than one answer)

SAHA is cytotoxic
SAHA affects cell viability
SAHA is antiproliferative, but not cytotoxic
SAHA triggers apoptosis
SAHA does not affect cell health
Check Your Answer

 

Drew Explains The Answer

Evaluating Mechanism of Action for SAHA

13160MA

Cells were treated with Camptothecin (topoisomerase inhibitor) at various concentrations for 48 hours. The mechanism of action was assessed using a multiplex of CellTox™ Green, CellTiter-Fluor™ and Caspase-Glo® Assays.

Interpret The Data

Based on the data, what can be determined about this compound's mechanism of action?

(Select more than one answer)

Camptothecin is cytotoxic
Camptothecin affects cell viability
Camptothecin affects cell proliferation
Camptothecin triggers apoptosis
Camptothecin does not affect cell health
Check Your Answer

 

Drew Explains The Answer

Evaluating Mechanism of Action for Camptothecin

FK866

Cells were treated with FK866 (NAD synthase inhibitor) at various concentrations for 48 hours. The mechanism of action was assessed using a multiplex of CellTox™ Green, CellTiter-Fluor™ and Caspase-Glo® Assays.

Interpret The Data

Based on the data, what can be determined about this compound's mechanism of action?

(Select more than one answer)

FK866 is cytotoxic
FK866 affects cell viability
FK866 is antiproliferative, but not cytotoxic
FK866 does not affect cell health
Check Your Answer

 

Drew Explains The Answer

Evaluating Mechanism of Action for FK866

Module 4 - Evaluating Antiproliferative vs. Cytotoxic Effects