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MagneSil® Total RNA mini-Isolation System

Fast, Automated RNA Isolation from Small Amounts of Cells, Tissue or Blood

  • Liquid handler-compatible
  • Concentrated RNA eluted in as little as 15μl

Size

Catalog number selected: Z3351

$ 1,152.00 Your price: Log In

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MagneSil® Total RNA mini-Isolation System
4 plate
$ 1,152.00
Your price: Log In

The MagneSil® Total RNA mini-Isolation System provides a high-throughput 96-well format for fast, simple preparation of intact total RNA from small amounts of cell culture (≤1 × 105 tissue culture cells), tissue (≤2mg tissue lysate in 100μl) or freshly isolated whole blood (≤20μl). The protocol enables high-throughput automated purification on a variety of liquid-handling workstations. Isolation of total RNA in a 384-well format from cell culture (≤1 × 103 cells) and freshly isolated whole blood (≤5μl) also may be performed. Total RNA purification is achieved without vacuum filtration, centrifugation or precipitation. The 96-well procedure takes about 30 minutes to complete using a liquid-handling workstation.

Total RNA purified using this system is suitable for a variety of molecular biology applications including endpoint RT-PCR amplification and real-time RT-PCR.

  • Only 30 minutes are required to process one 96-well plate, or 50 minutes for one 384-well plate on a Beckman Coulter Biomek® FX liquid handler.
  • Elution volumes as low as 15μl provide concentrated RNA without the need for time-consuming vacuum concentration.
  • DNase I treatment is included to remove genomic DNA contamination.
  • Validated automated methods are available at: www.promega.com/automethods/

Protocols

Specifications

What's in the box?

Item Part # SizeAvailable Separately

Nuclease-Free Water

P1195 1 × 150ml

RNA Lysis Buffer (RLA)

Z305D 1 × 100ml View Product

DNase Stop Solution

Z312D 1 × 26.5ml

Yellow Core Buffer

Z317D 1 × 25ml

MnCl2, 0.09M

Z318D 1 × 5ml

MagneSil® RNA Paramagnetic Particles

Z336D 1 × 20ml

DNase I (lyophilized)

Z358A 4 × 1 vial

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Patents and Disclaimers

U.S. Pat. Nos. 6,027,945 and 6,368,800, Australian Pat. No. 732756 and other patents and patents pending.

Resources

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