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GoTaq® Hot Start Master Mixes

M5122_GoTaq--Hot-Start-Green-Master-Mix-100rxn_3
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Hot-Start GoTaq® DNA Polymerase in a Ready-to-Use Master Mix

  • All-in-one master mix; just add template and primers
  • Minimize nonspecific amplification and primer-dimers
  • Use the green master mix for direct-to-gel analysis, and use the colorless master mix when post-amplification fluorescence or absorbance analysis is required

Choose a master mix

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Catalog number selected: M5122

$ 103.00
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GoTaq® Hot Start Master Mixes
Green/100 reactions
$ 103.00
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GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94-95 degrees C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70 degrees C. GoTaq® Hot Start Polymerase exhibits 5' --> 3' exonuclease activity. 

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. The Master Mixes contain a compound that increases sample density so that samples sink easily into wells of an agarose gel. The GoTaq® Hot Start Green Master Mix also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. Use the Green Master Mix for direct-to-gel analysis after amplification and the Colorless Master Mix for amplifications where the dyes may interfere with post-amplification analysis such as fluorescence or absorbance testing.

Specifications

You are viewing: M5122 Change Configuration

What's in the box?

Item Part # Size Available Separately

GoTaq® Hot Start Green Master Mix, 2X

M512B 2 × 1,250μl

Nuclease-Free Water

P119A 2 × 1,250μl View Product

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: M5123 Change Configuration

What's in the box?

Item Part # Size Available Separately

GoTaq® Hot Start Green Master Mix, 2X

M512C 1 × 25ml

Nuclease-Free Water

P119C 1 × 25ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: M5132 Change Configuration

What's in the box?

Item Part # Size Available Separately

GoTaq® Hot Start Colorless Master Mix, 2X

M513B 2 × 1,250μl

Nuclease-Free Water

P119A 2 × 1,250μl View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: M5133 Change Configuration

What's in the box?

Item Part # Size Available Separately

GoTaq® Hot Start Colorless Master Mix, 2X

M513C 1 × 25ml

Nuclease-Free Water

P119C 1 × 25ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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