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GoTaq® Hot Start Master Mixes

Hot-Start GoTaq® DNA Polymerase in a Ready-to-Use Master Mix

  • All-in-one master mix; just add template and primers
  • Minimize nonspecific amplification and primer-dimers
  • Use the green master mix for direct-to-gel analysis, and use the colorless master mix when post-amplification fluorescence or absorbance analysis is required

Choose a master mix

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Catalog number selected: M5122

$ 94.00
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GoTaq® Hot Start Master Mixes
Green/100 reactions
$ 94.00
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GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94-95 degrees C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70 degrees C. GoTaq® Hot Start Polymerase exhibits 5' --> 3' exonuclease activity. 

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. The Master Mixes contain a compound that increases sample density so that samples sink easily into wells of an agarose gel. The GoTaq® Hot Start Green Master Mix also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. Use the Green Master Mix for direct-to-gel analysis after amplification and the Colorless Master Mix for amplifications where the dyes may interfere with post-amplification analysis such as fluorescence or absorbance testing.

Specifications

You are viewing: M5122 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

GoTaq® Hot Start Green Master Mix, 2X

M512B 2 × 1,250μl

Nuclease-Free Water

P119A 2 × 1,250μl View Product

SDS

Choose language:

Download SDSPDF (42 KB) – English (United States)

Certificate of Analysis

See all certificates for M5122

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

Specifications

You are viewing: M5123 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

GoTaq® Hot Start Green Master Mix, 2X

M512C 1 × 25ml

Nuclease-Free Water

P119C 1 × 25ml View Product

SDS

Choose language:

Download SDSPDF (42 KB) – English (United States)

Certificate of Analysis

See all certificates for M5123

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

Specifications

You are viewing: M5132 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

GoTaq® Hot Start Colorless Master Mix, 2X

M513B 2 × 1,250μl

Nuclease-Free Water

P119A 2 × 1,250μl View Product

SDS

Choose language:

Download SDSPDF (42 KB) – English (United States)

Certificate of Analysis

See all certificates for M5132

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

Specifications

You are viewing: M5133 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

GoTaq® Hot Start Colorless Master Mix, 2X

M513C 1 × 25ml

Nuclease-Free Water

P119C 1 × 25ml View Product

SDS

Choose language:

Download SDSPDF (42 KB) – English (United States)

Certificate of Analysis

See all certificates for M5133

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.

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