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Save 20% on ReliaPrep™️ DNA & RNA Clean-Up and Concentration Systems through December 31st. Shop Now › 

SV 96 Total RNA Isolation System

Purifies Total RNA From 96 Samples in Under an Hour

  • Vacuum-based method. No centrifugation required
  • Plate design prevents cross-contamination during sample processing
  • Designed for both manual and automated RNA purification

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Catalog number selected: Z3500

$ 364.00
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SV 96 Total RNA Isolation System
1 × 96 each
$ 364.00
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Manual or High-Throughput RNA Purification. No Phenol, No Ethanol, No Fuss.

The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol:chloroform extraction or ethanol precipitation, and there is no detectable DNase carryover in the final RNA preparation.

Protocols are available for Beckman Coulter and PerkinElmer instruments.

  • Tested to ensure that purified RNA performs optimally in RT-PCR.
  • Novel vacuum manifold eliminates waste handling. Novel plate design prevents cross-contamination during sample processing.
  • Validated automated methods available at: www.promega.com/automethods/
  • Learn more about our custom options for this product at: www.promega.com/custom/

The SV 96 Total RNA Isolation System requires a vacuum pump capable of generating 15–20 inches of Hg or equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291; see figures) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).

For automated total RNA purification using the Biomek® 2000 Laboratory Automation Workstation, the Vacuum Manifold (Beckman Coulter Part# 609670) and Collar (Beckman Coulter Part# 609597) are required. See the Automated Protocol, #EP003, for additional information on automated RNA purification.

Specifications

You are viewing: Z3500 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Binding Plate

A227A 1 × 1 plate View Product

Elution Plate

A228A 1 × 1 plate

Plate Sealers

A230A 1 × 3 each

Nuclease-Free Water

P119E 1 × 13ml View Product

RNA Lysis Buffer (RLA)

Z3051 1 × 50ml View Product

RNA Wash Solution (RWA)

Z3091 1 × 58.8ml

DNase Stop Solution (DSA)

Z312C 1 × 13.25ml

Yellow Core Buffer

Z317A 1 × 2.5ml

MnCl2, 0.09M

Z318B 1 × 750μl

DNase I (lyophilized)

Z358A 1 × 1 vial

Beta-Mercaptoethanol

Z559A 1 × 900μl

SDS

Choose language:

Download SDSPDF (538 KB) – English (United States)

Certificate of Analysis

See all certificates for Z3500

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

See Protocol for detailed storage recommendations.

Specifications

You are viewing: Z3505 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Binding Plate

A227A 5 × 1 plate View Product

Elution Plate

A228A 5 × 1 plate

Plate Sealers

A230A 2 × 3 each

Nuclease-Free Water

P119E 5 × 13ml View Product

RNA Lysis Buffer (RLA)

Z3051 2 × 50ml View Product

RNA Wash Solution (RWA)

Z3091 5 × 58.8ml

DNase Stop Solution (DSA)

Z312C 5 × 13.25ml

Yellow Core Buffer

Z317A 5 × 2.5ml

MnCl2, 0.09M

Z318B 5 × 750μl

DNase I (lyophilized)

Z358A 5 × 1 vial

Beta-Mercaptoethanol

Z559A 2 × 900μl

SDS

Choose language:

Download SDSPDF (538 KB) – English (United States)

Certificate of Analysis

See all certificates for Z3505

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

See Protocol for detailed storage recommendations.

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