SV 96 Total RNA Isolation System

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Purifies Total RNA From 96 Samples in Under an Hour

  • Vacuum-based method. No centrifugation required
  • Plate design prevents cross-contamination during sample processing
  • Designed for both manual and automated RNA purification

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Catalog number selected: Z3500

$ 443.00
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SV 96 Total RNA Isolation System
1 × 96 each
$ 443.00
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Manual or High-Throughput RNA Purification. No Phenol, No Ethanol, No Fuss.

The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol:chloroform extraction or ethanol precipitation, and there is no detectable DNase carryover in the final RNA preparation.

Protocols are available for Beckman Coulter and PerkinElmer instruments.

  • Tested to ensure that purified RNA performs optimally in RT-PCR.
  • Novel vacuum manifold eliminates waste handling. Novel plate design prevents cross-contamination during sample processing.
  • Validated automated methods available at: www.promega.com/automethods/
  • Learn more about our custom options for this product at: www.promega.com/custom/

The SV 96 Total RNA Isolation System requires a vacuum pump capable of generating 15–20 inches of Hg or equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291; see figures) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).

For automated total RNA purification using the Biomek® 2000 Laboratory Automation Workstation, the Vacuum Manifold (Beckman Coulter Part# 609670) and Collar (Beckman Coulter Part# 609597) are required. See the Automated Protocol, #EP003, for additional information on automated RNA purification.

Specifications

You are viewing: Z3500 Change Configuration

What's in the box?

Item Part # Size Available Separately

Binding Plate

A227A 1 × 1 plate View Product

Elution Plate

A228A 1 × 1 plate

Plate Sealers

A230A 1 × 3 each

Nuclease-Free Water

P119E 1 × 13ml View Product

RNA Lysis Buffer (RLA)

Z3051 1 × 50ml View Product

RNA Wash Solution (RWA)

Z3091 1 × 58.8ml

DNase Stop Solution (DSA)

Z312C 1 × 13.25ml

Yellow Core Buffer

Z317A 1 × 2.5ml

MnCl2, 0.09M

Z318B 1 × 750μl

DNase I (lyophilized)

Z358A 1 × 1 vial View Product

Beta-Mercaptoethanol

Z559A 1 × 900μl

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

See Protocol for detailed storage recommendations.

Specifications

You are viewing: Z3505 Change Configuration

What's in the box?

Item Part # Size Available Separately

Binding Plate

A227A 5 × 1 plate View Product

Elution Plate

A228A 5 × 1 plate

Plate Sealers

A230A 2 × 3 each

RNA Lysis Buffer (RLA)

Z3051 2 × 50ml View Product

RNA Wash Solution (RWA)

Z3091 5 × 58.8ml

Beta-Mercaptoethanol

Z559A 2 × 900μl

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

See Protocol for detailed storage recommendations.

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