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RiboMAX™ Large Scale RNA Production Systems

Capped or Uncapped RNA Transcripts for in vitro or in vivo Translation

  • Produces 2–5mg/ml RNA in a 1ml reaction
  • Available for use with SP6 or T7 RNA polymerase
  • Scalable to meet varying RNA production requirements

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Catalog number selected: P1280

$ 328.00 Your price: Log In

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RiboMAX™ Large Scale RNA Production Systems
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$ 328.00
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More RNA from Transcription Reactions

The RiboMAX™ Large Scale RNA Production Systems consistently produce 2–5mg/ml of RNA in a 1ml reaction, about 10- to 20-fold more RNA than is produced with the standard Riboprobe® System transcription reaction. The RiboMAX™ System reactions differ from those of the Riboprobe® Systems in three main ways: a HEPES (pH 7.5) buffer is used rather than a Tris-HCl (pH 7.9) buffer; rNTP and magnesium concentrations are elevated at levels appropriate for either SP6 or T7 RNA polymerase; and inorganic pyrophosphatase is included in the reaction.

RNAs synthesized with the RiboMAX™ System perform better for in vitro translation in rabbit reticulocyte translation systems than RNA synthesized by standard methods. The reduction of components inhibitory to translation may be an advantage for other applications requiring biologically active RNA. Because the RiboMAX™ Systems produce large quantities of RNA, they are not recommended for generating high-specific-activity RNA probes.

Note: Use of the RiboMAX™ System for production of capped transcripts requires separate purchase of the Ribo m7G Cap Analog (Cat. P1711).

Applications of the RiboMAX™ Systems include:

  • Generation of large quantities of capped or uncapped RNA transcripts for in vitro or in vivo translation.
  • In vitro synthesis of tRNA, rRNA, RNA virus genomes and ribozymes.
  • Production of substrates for RNA splicing, RNA secondary structure, antisense RNA and RNA:protein interaction studies.
  • Synthesis of long RNAs for nonmammalian RNA interference.

References

  1. Gurevich, V.V. et al. (1991) Anal. Biochem. 195, 207–13.
  2. Beckler, G.S. (1992) Promega Notes 39, 12–6.

Protocols

Specifications

You are viewing: P1280 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

rATP, 100mM

E601D 1 × 100μl100mM View Product

rUTP, 100mM

E602D 1 × 100μl100mM View Product

rGTP, 100mM

E603D 1 × 100μl100mM View Product

rCTP, 100mM

E604D 1 × 100μl100mM View Product

SP6 Transcription Buffer, 5X

P131A 1 × 240μl

SP6 Linear Control DNA

P134A 1 × 10μl

Sodium Acetate, 3.0M (pH 5.2)

P135A 1 × 1ml

Enzyme Mix, SP6 RNA Polymerase

P133A 1 × 120μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

RQ1 RNase-Free DNase

M610C 1 × 110u View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: P1300 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

RQ1 RNase-Free DNase

M610C 1 × 110u View Product

rATP, 100mM

E601D 1 × 100μl100mM View Product

rUTP, 100mM

E602D 1 × 100μl100mM View Product

rGTP, 100mM

E603D 1 × 100μl100mM View Product

rCTP, 100mM

E604D 1 × 100μl100mM View Product

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Sodium Acetate, 3.0M (pH 5.2)

P135A 1 × 1ml

Enzyme Mix, T7 RNA Polymerase

P137A 1 × 120μl

T7 Transcription Buffer, 5X

P140A 1 × 240μl5X

T7 Linear Control DNA

P141A 1 × 10μl

SDS

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Certificate of Analysis

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View more results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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