RiboMAX™ Large Scale RNA Production Systems
Synthesizes Capped or Uncapped RNA Transcripts for in vitro or in vivo Translation
- Produces 2–5mg/ml RNA in a 1ml reaction
- Available for use with SP6 or T7 RNA polymerase
- Scalable to meet varying RNA synthesis needs
- Animal origin-free (AOF) and cGMP-manufactured version available for RNA therapeutic applications. Learn more.
Catalog Number:
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More RNA from Transcription Reactions
The RiboMAX™ Large Scale RNA Production System generate large quantities of capped or uncapped RNA for in vitro or in vivo translation. RNA synthesized with this system performs better for in vitro rabbit reticulocyte translation systems than RNA synthesized by standard methods, particularly at high concentrations that usually inhibit translation.
Key Benefits
- High yield: Consistently produces 10 to 20-fold more RNA than the standard Riboprobe® System.
- Translation-ready RNA: Reduced components that inhibit in vitro translation; better results for applications requiring biologically active RNA.
- Flexible output: Produce capped or uncapped transcripts for a wide range of downstream applications. (Note: Production of capped transcripts requires separate purchase of the Ribo m7G Cap Analog)
Effect of pGEM®-luc RNA concentration on in vitro translation. The indicated amounts of pGEM®-luc RNA transcripts were used to program 50µl of Rabbit Reticulocyte Lysate in vitro translation reactions. Expression of functional luciferase was measured as follows: 2.5µl of each reaction was mixed with an equal volume of Luciferase Assay Reagent and luminescence measured for 10 seconds in a luminometer.
RiboMax™ vs. Riboprobe® Systems
| Product | RiboMAX™ Large Scale RNA Production System | Riboprobe® System |
|---|---|---|
| RNA yield | 10 to 20-fold more RNA than Riboprobe® System | Lower yield |
Buffer |
HEPES (pH 7.5) | Tris-HCl (pH 7.9) |
rNTP & Mg²⁺ levels |
Elevated levels appropriate for SP6 or T7 RNA polymerase | Standard levels |
Inorganic pyrophosphatase |
Included | Not included |
| Recommended use | Large-scale synthesis. Not recommended for generating high-specific-activity radiolabeled RNA probes (due to radiolabeled nucleotide cost at high yield). | Standard synthesis |
Applications
- Generation of dsRNA and long RNAs for nonmammalian RNA interference studies.
- In vitro synthesis of tRNA and rRNA, and RNA virus genomes and ribozymes.
- Production of substrates for RNA splicing, studies of RNA secondary structure and RNA:protein interactions.
- Synthesis of antisense RNA
Frequently-Asked Questions
Which polymerases are supported?
SP6 and T7 RNA polymerases; reaction conditions are optimized for each.
Can I make capped RNA?
Yes. Add Ribo m7G Cap Analog (Cat.# P1711) for capped transcripts.
Why does it perform better at high RNA concentrations in translation assays?
Formulation reduces components that typically inhibit rabbit reticulocyte translation, supporting efficient protein synthesis even with higher RNA inputs.
How does it compare to Riboprobe® Systems?
Typical yields are about 10- to 20-fold higher, with a formulation optimized for large-scale RNA production.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
rATP, 100mM |
E601D | 1 × 100μl | 100mM | View Product |
|
rUTP, 100mM |
E602D | 1 × 100μl | 100mM | View Product |
|
rGTP, 100mM |
E603D | 1 × 100μl | 100mM | View Product |
|
rCTP, 100mM |
E604D | 1 × 100μl | 100mM | View Product |
|
SP6 Transcription Buffer, 5X |
P131A | 1 × 240μl | ||
|
SP6 Linear Control DNA |
P134A | 1 × 10μl | ||
|
Sodium Acetate, 3.0M (pH 5.2) |
P135A | 1 × 1ml | ||
|
Enzyme Mix, SP6 RNA Polymerase |
P133A | 1 × 120μl | ||
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product | |
|
RQ1 RNase-Free DNase |
M610C | 1 × 110u | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
RQ1 RNase-Free DNase |
M610C | 1 × 110u | View Product | |
|
rATP, 100mM |
E601D | 1 × 100μl | 100mM | View Product |
|
rUTP, 100mM |
E602D | 1 × 100μl | 100mM | View Product |
|
rGTP, 100mM |
E603D | 1 × 100μl | 100mM | View Product |
|
rCTP, 100mM |
E604D | 1 × 100μl | 100mM | View Product |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product | |
|
Sodium Acetate, 3.0M (pH 5.2) |
P135A | 1 × 1ml | ||
|
Enzyme Mix, T7 RNA Polymerase |
P137A | 1 × 120μl | ||
|
T7 Transcription Buffer, 5X |
P140A | 1 × 240μl | 5X | |
|
T7 Linear Control DNA |
P141A | 1 × 10μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Featured Resource
High Volume In Vitro Transcription Reactions for RNA Synthesis
Learn how to synthesize large quantities of RNA with up to 10ml of in vitro transcription reactions prepared using the RiboMAX™ Large Scale RNA Production System.
Resources
Other Resources
- High-Volume In Vitro Transcription Reactions for RNA Synthesis Application Note
- Agilent Application Note: Assessment of Long IVT mRNA Fragments with the Agilent 5200 Fragment Analyzer System
- Product Application: Automated Purification of RNA Transcripts
- Product Application: Synthesis of Circular RNAs Using the RiboMAX™ System
- Development of mRNA manufacturing for vaccines and therapeutics: mRNA platform requirements and development of a scalable production process to support early phase clinical trials
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